Journal: Scientific Reports

Article Title: Seroprevalence of six pathogens transmitted by the Ixodes ricinus ticks in asymptomatic individuals with HIV infection and in blood donors

doi: 10.1038/s41598-019-38755-9

Figure Lengend Snippet: Prevalence of anti- B . burgdorferi , anti- B . microti , anti- A . phagocytophilum , anti- Ehrlichia spp., anti- B . henselae and anti- Rickettsia spp. IgM and IgG antibodies in HIV-infected patients and blood donors in Poland.

Article Snippet: ; (2) Western Blot: recomLine Borrelia IgM and recomLine Borrelia IgG (Microgen, Neuried, Germany) for positive or doubtful results of Borrelia ELISA test for confirmation according to the European guidelines (Stanek et al . 2011); (3) Ehrlichia chaffeensis and Anaplasma phagocytophilum IFA IgM Antibody Kit (Fuller Laboratories, California, the USA; positive cut-off 1/512) for A. phagocytophilum i Ehrlichia spp.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Seroprevalence of six pathogens transmitted by the Ixodes ricinus ticks in asymptomatic individuals with HIV infection and in blood donors

doi: 10.1038/s41598-019-38755-9

Figure Lengend Snippet: The frequency of doubtful results in HIV-infected patients and blood donors in Poland.

Article Snippet: ; (2) Western Blot: recomLine Borrelia IgM and recomLine Borrelia IgG (Microgen, Neuried, Germany) for positive or doubtful results of Borrelia ELISA test for confirmation according to the European guidelines (Stanek et al . 2011); (3) Ehrlichia chaffeensis and Anaplasma phagocytophilum IFA IgM Antibody Kit (Fuller Laboratories, California, the USA; positive cut-off 1/512) for A. phagocytophilum i Ehrlichia spp.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Medicine

Article Title: Development of a New Genus-Specific Quantitative Real-Time PCR Assay for the Diagnosis of Scrub Typhus in South America

doi: 10.3389/fmed.2022.831045

Figure Lengend Snippet: DNA preparations from bacterial strains and other sources included in the validation of the novel qPCR assay, Orien16S.

Article Snippet: Citrano (Australia) , , , Ehrlichia chaffeensis , .

Techniques: Biomarker Discovery

Journal: PLoS ONE

Article Title: Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response

doi: 10.1371/journal.pone.0205983

Figure Lengend Snippet: (A) E . chaffeensis- infected and uninfected control THP-1 cells were fixed and probed with mouse anti-TRP47 (green), rabbit anti-Dsb (red), and DAPI (blue), and visualized by scanning confocal laser microscopy. For each timepoint post-infection, one representative optical slice from the z stack is shown. TRP47 was detected in all three timepoints in the infected cells. TRP47 was mainly found in cytoplasm at 24 hpi (in and around the morulae), whereas increased TRP47 was detected in the nucleus at 48 and 72 hpi. Ehrlichial Dsb (red) in micrographs confirmed presence of E . chaffeensis in infected cells at 24, 48 and 72 hpi. We did not observe staining for TRP47 or Dsb in uninfected control. (B) Orthogonal projections of optical slices from a z-stack confirmed the presence of TRP47 in the nucleus especially at 48 and 72 hpi. Top panels show an x-z projection and right panels show a y-z projection. The positions of the x and y axes within the projections denote the z depth of the slice shown in the center.

Article Snippet: PCR products were generated from E . chaffeensis genomic DNA with HotMasterMix (5 PRIME, Gaithersburg, Md.) using the following thermal cycling program: 94°C for 2 min; 30 cycles of 94°C for 30 s, annealing temperature (5°C less than the lowest primer Tm) for 30 s, and 65°C for the appropriate extension time (30 s per 500 product base pairs); and 65°C for 7 min.

Techniques: Infection, Control, Microscopy, Staining

Journal: PLoS ONE

Article Title: Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response

doi: 10.1371/journal.pone.0205983

Figure Lengend Snippet: (A) TRP47 5 tandem repeat (5TR) construct binds to host genomic DNA in EMSA. Incubation of biotin-labeled host gDNA with 4 μg TRP47 5TR (lane 2) yielded a strong shifted band (band height marked by arrow) compared to labeled gDNA alone (lane 1). Binding was prevented with addition of excess unlabeled competitor gDNA (lane 3), and with high concentration of anti-TRP47 antibody (lanes 4–7 are with 1:20, 1:50, 1:100, and 1:200, respectively). These lanes were rearranged from the original blot and white lines denote lanes that were not adjacent. (B) TRP47 full-length (FL) construct binds to host genomic DNA in EMSA. Incubation of labeled gDNA with 8 μg TRP47 FL (lane 3) yielded a shifted band (marked by arrow) not present with labeled gDNA alone (lane 1) nor with 4 μg TRP47 FL (lane 2). Binding was prevented with addition of excess unlabeled competitor gDNA (lane 4). A different gDNA preparation was used for the EMSA in panel B likely accounting for the difference in free gDNA migration and background.

Article Snippet: PCR products were generated from E . chaffeensis genomic DNA with HotMasterMix (5 PRIME, Gaithersburg, Md.) using the following thermal cycling program: 94°C for 2 min; 30 cycles of 94°C for 30 s, annealing temperature (5°C less than the lowest primer Tm) for 30 s, and 65°C for the appropriate extension time (30 s per 500 product base pairs); and 65°C for 7 min.

Techniques: Construct, Incubation, Labeling, Binding Assay, Concentration Assay, Migration

Journal: PLoS ONE

Article Title: Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response

doi: 10.1371/journal.pone.0205983

Figure Lengend Snippet: E . chaffeensis TRP47 and TRP120 common target genes.

Article Snippet: PCR products were generated from E . chaffeensis genomic DNA with HotMasterMix (5 PRIME, Gaithersburg, Md.) using the following thermal cycling program: 94°C for 2 min; 30 cycles of 94°C for 30 s, annealing temperature (5°C less than the lowest primer Tm) for 30 s, and 65°C for the appropriate extension time (30 s per 500 product base pairs); and 65°C for 7 min.

Techniques: Translocation Assay, Cell Differentiation, Activity Assay

Journal: PLoS ONE

Article Title: Ehrlichia chaffeensis TRP47 enters the nucleus via a MYND-binding domain-dependent mechanism and predominantly binds enhancers of host genes associated with signal transduction, cytoskeletal organization, and immune response

doi: 10.1371/journal.pone.0205983

Figure Lengend Snippet: E . chaffeensis TRP32, TRP47, and TRP120 common target genes.

Article Snippet: PCR products were generated from E . chaffeensis genomic DNA with HotMasterMix (5 PRIME, Gaithersburg, Md.) using the following thermal cycling program: 94°C for 2 min; 30 cycles of 94°C for 30 s, annealing temperature (5°C less than the lowest primer Tm) for 30 s, and 65°C for the appropriate extension time (30 s per 500 product base pairs); and 65°C for 7 min.

Techniques: Cell Differentiation, Transduction, Binding Assay, Membrane, Modification